Journal: PeerJ

Article Title: DNA mismatch repair and CD133-marked cancer stem cells in colorectal carcinoma

doi: 10.7717/peerj.5530

Figure Lengend Snippet: (A) The weak CD133 staining seen at the luminal border of some normal colonic crypts is considered equivocal and interpreted as negative (x200). In contrast, (B) is a case of colorectal carcinoma with strong CD133 staining of the glandular luminal surface (x200) and (C) a case of poorly differentiated colorectal carcinoma demonstrating CD133 staining as tiny cytoplasmic blobs which represent abortive glands (x200). (D) Colorectal carcinoma with deficient DNA mismatch repair (represented by loss of MLH1). Note the lack of immunoexpression of MLH1 in the colorectal carcinoma nuclei in the presence of immunopositivity in the internal positive controls (stromal cells, lymphocytes and nearby normal enterocytes) (x200)

Article Snippet: Immunohistochemical staining with a rabbit polyclonal antibody to CD133 (1:200; Abcam ab19898, Cambridge, United Kingdom) and detection by the ultraView universal DAB detection kit (Ventana Medical Systems Inc., Tucson, AZ, USA) was carried out on the Ventana Benchmark XT autostainer (Ventana Medical Systems Inc., Tucson, AZ, USA).

Techniques: Staining

Journal: PeerJ

Article Title: DNA mismatch repair and CD133-marked cancer stem cells in colorectal carcinoma

doi: 10.7717/peerj.5530

Figure Lengend Snippet: CD133 expression and DNA mismatch repair (MMR) status. The CD133 expression and MMR status as per size, TNM stage and differentiation of the colorectal carcinoma ( n = 80).

Article Snippet: Immunohistochemical staining with a rabbit polyclonal antibody to CD133 (1:200; Abcam ab19898, Cambridge, United Kingdom) and detection by the ultraView universal DAB detection kit (Ventana Medical Systems Inc., Tucson, AZ, USA) was carried out on the Ventana Benchmark XT autostainer (Ventana Medical Systems Inc., Tucson, AZ, USA).

Techniques: Expressing

Journal: PeerJ

Article Title: DNA mismatch repair and CD133-marked cancer stem cells in colorectal carcinoma

doi: 10.7717/peerj.5530

Figure Lengend Snippet: DNA mismatch repair protein (MMR) status and CD133 expression. CD133 immunoexpression and score being stratified by MMR status in right-sided and left-sided colorectal carcinomas.

Article Snippet: Immunohistochemical staining with a rabbit polyclonal antibody to CD133 (1:200; Abcam ab19898, Cambridge, United Kingdom) and detection by the ultraView universal DAB detection kit (Ventana Medical Systems Inc., Tucson, AZ, USA) was carried out on the Ventana Benchmark XT autostainer (Ventana Medical Systems Inc., Tucson, AZ, USA).

Techniques: Expressing

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Effect of Shogaol on the Expression of Intestinal Stem Cell Markers in Experimentally Induced Colitis in BALB/c Mice

doi: 10.1155/2019/5134156

Figure Lengend Snippet: Cytoplasmic and membranous CD133 expression in epithelial cells of the colonic crypts. (a) Minimal, week expression (sum score 0) in mice of control negative group. (b) Focal, weak expression (sum score 1) in control positive group. (c) Focal, weak-moderate expression (sum score 4) deeply within the colonic crypt in group 3 (DSS exposure & 6-TG treatment). (d) Diffuse, weak expression (sum score 3) in group 4 (DSS exposure & 10 mg/kg b.w. Shogaol treatment). (e) and (f) Diffuse, moderate expression (sum score 6) extends into the transient amplifying and apical regions of the colonic crypts in group 5 (DSS exposure & 20 mg/kg b.w. Shogaol treatment) and group 6 (DSS exposure & 40 mg/kg b.w. Shogaol treatment), respectively.

Article Snippet: Following that, the slides were divided into 2 sets: the first set was incubated overnight at 4°C with rabbit anti-CD133 polyclonal Abs (1 : 100, San Francisco Biorbyt, USA), and the second set was incubated with rabbit anti-CD34 monoclonal Abs (1 : 100, Dako, Germany).

Techniques: Expressing, Control

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Effect of Shogaol on the Expression of Intestinal Stem Cell Markers in Experimentally Induced Colitis in BALB/c Mice

doi: 10.1155/2019/5134156

Figure Lengend Snippet: Immunofluorescence expression of the CD133 protein in the cytoplasm of the colonic cryptal epithelium. (a) and (b) Weak (+) expression in mice of the control negative and control positive group. (c) and (d) Weak-moderate (+ to ++) expression in the in deep portions of the colonic crypts in mice of group 3 (6-TG treatment) and group 4 (10 mg/kg b.w. Shogaol treatment). (e) and (f) Moderate (++) expression in mice of group 5 (20 mg/kg b.w. Shogaol treatment) and mice of group 6 (40 mg/kg b.w. Shogaol treatment).

Article Snippet: Following that, the slides were divided into 2 sets: the first set was incubated overnight at 4°C with rabbit anti-CD133 polyclonal Abs (1 : 100, San Francisco Biorbyt, USA), and the second set was incubated with rabbit anti-CD34 monoclonal Abs (1 : 100, Dako, Germany).

Techniques: Immunofluorescence, Expressing, Control